Innovative application of mass spectrometry for the characterization of staphylococcal enterotoxins involved in food poisoning outbreaks.
نویسندگان
چکیده
Staphylococcal poisoning is a common food-borne disease for which immunoassays to detect enterotoxins were developed, but these assays often lead to false diagnoses due to interferences or lack of specificity. Absolute quantitative mass spectrometry was for the first time successfully applied to an investigation of a staphylococcal outbreak due to coconut pearls.
منابع مشابه
How Should Staphylococcal Food Poisoning Outbreaks Be Characterized?
Staphylococcal food poisoning is one of the most common food-borne diseases and results from the ingestion of staphylococcal enterotoxins (SEs) preformed in food by enterotoxigenic strains of Staphylococcus aureus. To date, more than 20 SEs have been described: SEA to SElV. All SEs have superantigenic activity whereas only a few have been proved to be emetic, representing a potential hazard for...
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Staphylococcal food poisoning represents the most prevalent foodborne intoxication worldwide. It is caused by oral intake of enterotoxins preformed by Staphylococcus aureus in food. The relevance of newly described enterotoxins in outbreaks of staphylococcal food poisoning is controversially discussed. Although the staphylococcal enterotoxins SEG, SEI, SEM, SEN, and SEO elicit emesis in a monke...
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Staphylococcal enterotoxins are major causing agents of food-borne diseases. Their detection in food remnants for risk assessment or food poisoning outbreaks investigation suffers from a lack in comprehensive immunological tools. In this study, we demonstrate that the combination of immunocapture and Protein Standard Absolute Quantification (PSAQ) strategy, which uses isotope-labeled enterotoxi...
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Methods are described for the extraction and serological detection of trace amounts of enterotoxins A and B in foods incriminated in outbreaks of staphylococcal food poisoning. Evidence is presented for the probable applicability of the methods for the detection of unidentified enterotoxins.
متن کاملStaphylococcus Aureus
To date, 21 different staphylococcal enterotoxins have been described (known by letters of the alphabet, A V, although a few letters are missing from the sequence). All are heat stable, water-soluble proteins and retain their activity in the digestive tract after ingestion. Staphylococcal enterotoxin A (SEA), either alone, or in combination with other staphylococcal enterotoxins, is the one mos...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 75 3 شماره
صفحات -
تاریخ انتشار 2009